![]() ![]() Use the scrambler tool to randomly shuffle peptide or protein sequences.Ĭopyright © 2012-2023. Try the sequence splitter tool for fragmenting sequences of proteins and peptides with optional overlap. The molecular weight is directly related to the length of the amino acid sequence and is expressed in units called daltons (Da). Peptide with Phosphoserine: Arg-Arg-Ala-Ser(PO3)-Pro-Val-Ala Also on this site Peptide with isotopically labeled Valine and Isoleucine: Peptide with disulfide bridge (1-6), capped at C-terminal (amide): Cyclic CYFQNCPRG-NH2 Peptide with Biotin on N-terminal: Biotin-YGRKKRRQRRR point (pi) and molecular weight (MW) using simply the amino acid sequence of the protein would be extremely valuable to researchers who use two-dimensional. Uses the module IsoelectricPoint to calculate the pI of a protein. The pKa value of Amino acids depends on its. Compute pI/Mw is a tool which allows the computation of the theoretical pI (isoelectric point) and Mw (molecular weight) for a list of Swiss-Prot and/or TrEMBL. Alignments: Pairwise DNA Alignment: Align two DNA sequences and color the resulting global alignment. Reverse Complement: Convert a DNA sequence into its reverse, complement, or reverse-complement. Protein pI is calculated using pKa values of amino acids. DNA Molecular Weight: Calculate the molecular weight of DNA single-stranded sequences are counted with a 5-P. Peptide with two disulfide bridges: ACDCRGDCFCG Where (for proteins in water measured at 280 nm): Ext(Tyr) 1490, Ext(Trp) 5500, Ext(Cystine) 125 The absorbance (optical density) can be calculated using the following formula: Absorb(Prot) E(Prot) / Molecularweight. Head-to-tail cyclic peptide: cyclo(GRGDSP) pI Charge isoelectric( SeqAA ) returns the estimated isoelectric point ( pI ). Linear peptide in three-letter code: Arg-Gly-Asp-Cys The isoelectric point is the pH at which the protein has a net charge of zero. Linear peptide in one-letter code: KYICNSSCM Please note: this is a rapidly changing project in development look, feel, and functionality may and will change. Take a look at the following posts for demonstration of what the calculator is capable of:įor a quick sample of the output screen, you can load a random peptide from the list of peptides the analytical tool has calculated before. The peptide molecular weight calculator will display average and monoisotopic mass of the molecule, as well as a table of mass divided by charge values, both in positive and negative scan modes, which is useful for mass spec analysis. Simply type in, or copy and paste, peptide or protein fragment amino-acid sequence, including modifications, spacers, or special termini, and press the “Calculate” button. Sequence How to use this peptide analytical tool ![]()
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